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| Registros recuperados: 31 | |
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| Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés. |
| Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis... |
| Tipo: Journal article |
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012 |
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| Delcroix, Jeremy; Gatesoupe, F.j.; Desbruyeres, Elisabeth; Huelvan, Christine; Le Delliou, Herve; Le Gall, Marie-madeleine; Quazuguel, Patrick; Mazurais, David; Zambonino-infante, Jose-luis. |
| Protein hydrolysate is an essential component of dry starter diets for fish larvae, as promoting healthy development. Peptides are also suitable substrates for many intestinal microbes. Five experimental diets were compared to a control diet (CONT) supplemented with a commercial fish protein hydrolysate. Each diet contained one marine protein hydrolysate, which differed by the proportion of di- and tri-peptides, and by raw materials. Two diets (HYD4 and HYD5) stimulated larval growth compared with CONT. Two other diets (HYD1 and HYD2) yielded inferior growth. HYD1 was detrimental for survival, which was associated with an up-regulation of genes involved in inflammation and antioxidative responses, while the gene coding for osteocalcin was down-regulated in... |
| Tipo: Text |
Palavras-chave: Compound diets; Denaturing gradient gel electrophoresis; Digestive enzymes; Gene expression; Gut maturation; Peptides. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00197/30865/29268.pdf |
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| Nguyen, Thi-my-huong. |
| The main objective of this study is the valorisation of by-products coming from processed tuna (Thunnus albacares). Therefore, enzymatic hydrolysis was selected, using an industrial large spectrum protease (Protamex). In a first phase, hydrolyses of these by-products (heads, tails, viscera) were carried out for long periods of time (12h) in order to establish the kinetics of the proteolysis of those different by-products and to obtain the approximate composition of the different resulting fractions and to determine the conditions which would allow the valorisation of these fractions. In a second phase the valorisation in animal nutrition (shrimp culture) and in human nutrition (fish sauce) of the fractions coming from the hydrolysis of the head of tunas... |
| Tipo: Text |
Palavras-chave: Feed; Food; Lipids; Peptids; Protamex; Proteolysis; By products; Thuna; Alimentation; Lipides; Peptides; Protamex; Protéolyse; Co produits; Thon. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/these-6918.pdf |
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| Holford, Mande; Puillandre, Nicolas; Terryn, Yves; Cruaud, Corinne; Olivera, Baldomero; Bouchet, Philippe. |
| Toxoglossate marine gastropods, traditionally assigned to the families Conidae, Terebridae, and Turridae, are one of the most populous animal groups that use venom to capture their prey. These marine animals are generally characterized by a venom apparatus that consists of a muscular venom bulb and a tubular venom gland. The toxoglossan radula, often compared with a hypodermic needle for its use as a conduit to inject toxins into prey, is considered a major anatomical breakthrough that assisted in the successful initial radiation of these animals in the Cretaceous and early Tertiary. The pharmacological success of toxins from cone snails has made this group a star among biochemists and neuroscientists, but very little is known about toxins from the other... |
| Tipo: Text |
Palavras-chave: Life history similarities; Similar proboscis type; Morphology; Biology; Peptides; Radula. |
| Ano: 2009 |
URL: https://archimer.ifremer.fr/doc/00207/31788/30197.pdf |
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| Mitta, Guillaume. |
| Les mécanismes immunitaires innés des vertébrés sont une mosaïque de mécanismes ancestraux partagés avec des animaux évolutivement plus primitifs. Les peptides antimicrobiens sont une de ces composantes conservées au travers du règne animal. En effet, ils participent vraisemblablement chez tous les métazoaires aux processi d'immunité innée. Toutefois, leurs implications dans la réponse anti-infectieuse peuvent être assez différentes. L'objet de cette thèse était de comprendre comment ces peptides participaient aux processi de défense de la moule. Une première phase de caractérisation biochimique de telles molécules avait été entreprise chez la moule, Mytilus galloprovincialis, dans le cadre de la thèse de Florence Hubert. Notre travail a consisté (i) à... |
| Tipo: Text |
Palavras-chave: Immunité; Peptides; Antibiotiques; Moule; Défensine. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00439/55101/56547.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio. |
| Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in... |
| Tipo: Text |
Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf |
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| Bernay, Benoît. |
| Despite the fact that the involvement of environmental factors in the physiology of the japanese oyster C. gigas is fully described, regulatory peptides are not known. In this study, we focused on peptidergic control of egg-laying. In order to characterize peptides involved in the regulation of egg-laying in C. gigas, the cuttlefish S. officinalis was used as a model. Firstly, regulatory peptides were investigated in the cuttlefish by peptidomic analysis. This approach led to the identification of eleven new ovarian regulatory peptides. Based on conserved tags, these peptides have been classified into two families: the SepCRPs (Sepia Capsule Releasing Peptides) and the OJPs (Ovarian Jelly Peptides). Released by oocytes into the genital coelom, they are... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Ovaire; Spectrométrie de masse; Peptides; Céphalopodes; Bivalves; Mollusques. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/these-2566.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis. |
| The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first... |
| Tipo: Text |
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf |
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| Vonorax,E. J.; Tyler,M. I.; Atkinson,R. K.; Howden,M. E. H.. |
| Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Australian funnel-web spiders; Hadronyche and Atrax species; Venom; Insecticidal toxins; Peptides; Helicoverpa armigera. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200005 |
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| Sahayaraj,K.; Borgio,J. F.; Muthukumar,S.; Anandh,G. P.. |
| The reduviid predators Rhynocoris marginatus (Fab.) and Catamirus brevipennis (Servile) use their venoms to paralyze their preys. We detected the antibacterial activity of R. marginatus and C. brevipennis venoms against seven Gram-negative and four Gram-positive bacteria by using the disc diffusion method. Rhynocoris marginatus venom exhibited antibacterial activity against four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium) and one Gram-positive (Streptococcus pyogenes). Catamirus brevipennis venom showed antibacterial activity against six Gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium) and three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibacterial activity; Reduviid saliva; Protein content; Peptides. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300011 |
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| Barros,Gustavo A. C.; Pereira,Andreia V.; Barros,Luciana C.; Lourenço Jr,Airton; Calvi,Sueli A.; Santos,Lucilene D.; Barraviera,Benedito; Ferreira Jr,Rui Seabra. |
| Abstract Background American visceral leishmaniasis is caused by the intracellular parasiteLeishmania (L.) infantum chagasi, and transmitted by the sand fly Lutzomyia longipalpis. Since treatment is based on classical chemotherapeutics with significant side effects, the search for new drugs remains the greatest global challenge. Thus, this in vitro study aimed to evaluate the leishmanicidal effect ofCrotalus durissus terrificus venom fractions on promastigote and amastigote forms of Leishmania (L.) infantum chagasi. Methods Phospholipase A 2 (PLA 2 ) and a pool of peptide fraction (<3 kDa) were purified from Crotalusvenom. Furthermore, promastigotes and peritoneal macrophages of mice infected by amastigotes were exposed to serial dilutions of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PLA2; Peptides; Crotalus dutissus terrificus; Venom; Leishmanicidal activity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100351 |
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| Rodríguez-Ravelo,Rodolfo; Coronas,Fredy I V; Zamudio,Fernando Z; González-Morales,Lidia; López,Georgina Espinosa; Urquiola,Ariel Ruiz; Possani,Lourival D. |
| Backgound The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country. Results Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cuba; Hyaluronidase; Peptides; Phospholipase; Proteomics; Rhopalurus junceus; Scorpion. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100309 |
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| Lima,P. R. de; Brochetto-Braga,M. R.. |
| Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hymenoptera; Venom; Enzymes; Peptides. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200002 |
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| Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito. |
| Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301 |
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| 三上, 正幸; 島田, 謙一郎; 関川, 三男; MIKAMI, Masayuki; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo. |
| Some properties of the non-heated meat products in European countries were analyzed. The dry fermented sausages used were Spanish salami (small，medium and large size)，Chorizo， Pepper salami， Italian salami，Hungarian salami and Serrano ham. Viable bacterial counts were small number (10³-10⁵CFU/g) in the hard type sausages and were large number (10⁷ -10⁸CFU/g) in the semi soft type sausages. The pH of the dry fermented sausages inoculated with mold was 5.4-6.7 and was higher than 5.1 of Chorizo which was not inoculated with mold. Peptides and total free amino acids content were 634.0 - 2，240.7 mg/100g products and 404.8 - 2，413.4 mg/100g products， respectively. These were high numbers in Serrano ham. Nitrite ion contents were low level (0.3-4.3 ppm) in... |
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Palavras-chave: 発酵ソーセージ; 乾塩ハム; ペプチド; 遊離アミノ酸; Dry fermented sausages; Dry cured ham; Peptides; Free amino acids. |
| Ano: 2001 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2992 |
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| Silva,Thiberio Carvalho da; Rocha,Joana D’Arc Mauricio; Moreira,Pedro; Signor,Altevir; Boscolo,Wilson Rogerio. |
| Abstract: The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cetengraulis edentulus; Oreochromis niloticus; Digestibility; Fish meal; Nutrition; Peptides; Pisciculture.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000700485 |
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| Registros recuperados: 31 | |
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